Lb. Masters et al., A CLINICAL-EVALUATION OF DEMINERALIZED FREEZE-DRIED BONE ALLOGRAFT INCOMBINATION WITH TETRACYCLINE IN THE TREATMENT OF PERIODONTAL OSSEOUSDEFECTS, Journal of periodontology, 67(8), 1996, pp. 770-781
THE PURPOSE WAS TO EVALUATE the use of demineralized freeze-dried bone
allograft reconstituted with 50 mg/ml tetracycline hydrochloride in t
he treatment of intrabony periodontal defects. Fifteen systemically he
althy patients (12 females, 3 males; aged 35 to 61) with moderate-adva
nced periodontitis were treated. Patients had 3 osseous defects with p
robing depths (PD) > 5 mm after initial therapy. Each site in each sub
ject was randomly assigned to one of the following groups: 1) deminera
lized freeze-dried bone allograft reconstituted with 50 mg/ml tetracyc
line (DFDBA + TCN); 2) demineralized freeze-dried bone allograft alone
(DFDBA); or 3) debridement only (D). Clinical measurements were taken
the day of surgery, 6 months, and 1 year. Standardized radiographs we
re taken at baseline and 1 year and were evaluated by computer assiste
d densitometric image analysis (CADIA). Clinical measurements included
gingival recession, PD, clinical attachment level, and mobility. Osse
ous defect measurements were taken at baseline and at the 1 year reent
ry, No adverse healing responses occurred. The results showed that ail
patients had a statistically significant improvement in probing depth
and attachment Level at 1 year, Osseous measurements showed bone fill
of 2.27 mm (51.6%) for the DFDBA + TCN group, 2.20 mm (52.4%) for the
DFDBA group, and 1.27 mm (32.8%) for the D group. Defect resolution w
as 77.3%; for the DFDBA + TCN group, 77.9% for the DFDBA group, and 63
.8% for the D group, The mean CADIA values were 5.01 fur the DFDBA + T
CN group, 6.79 for the DFDBA group and 2.78 for tile D group. The CADI
A values did not correlate with the clinical parameters. Although the
grafted groups showed greater bone fill and defect resolution, there w
as no statistically significant difference in any of the clinical para
meters between the treatment groups. This study suggests that there is
no significant benefit from reconstituting the allograft with 50 mg/m
l of tetracycline hydrochloride.