CALVING OUTCOME FOLLOWING TRANSFER OF EMBRYOS PRODUCED IN-VITRO IN DIFFERENT CONDITIONS

A survey of calving outcome from embryos produced in vitro by three la boratories under different culture conditions is presented. A total of 572 embryos were transferred after culture and 170 calves were born: 9 calves from 47 (19%) embryos produced in coculture with bovine ovidu ct cells (BOCC) or medium conditioned by these cells (BOCM+); 5 from 1 6 (31%) embryos produced in serum free oviduct conditioned medium (BOC MS-); 8 from 24 (33%) embryos derived from BRL conditioned medium (BRL CM-); 0 from 9 embryos cultured in SOF (20% O-2); 132 from 427 (31%) c ocultured with granulosa cells (GCC); 16 from 49 (33%) embryos culture d in SOF (5% O-2). Frozen embryos (n = 120) were transferred soon afte r thawing and 25 calves were obtained: ii from 59 (19%) embryos in the first condition (BOCC); 6 from 19 (32%) in the second condition (BOCM S+); 6 from 34 (18%) in the third (BOCMS-) and 2 from 8 (25%) in the f ourth condition (BRLCMS-). Gestation length and birth weight were in t he normal range except for 5 calves that were exceptionally large. Sex ratio was in favour of males. Neo and perinatal mortality ranged from O to 19%. Three cases of malformations were observed, Growth rate was normal.