X. Zuo et Ptk. Woo, ACID-PHOSPHATASE IN THE PATHOGENIC AND NONPATHOGENIC HEMOFLAGELLATES,CRYPTOBIA SPP, OF FISHES, The Journal of parasitology, 82(6), 1996, pp. 893-899
Acid phosphatase (ACP) was detected in whole-cell lysates, membrane-bo
und and water-soluble fractions of Cryptobia salmositica (pathogenic a
nd nonpathogenic vaccine strains), Cryptobia bullocki, and Cryptobia c
atostomi using p-nitrophenylphosphate as the substrate. High activitie
s were in acidic pH (3.0-5.5) and the optimal pH was 5.0. Highest ACP
activity was in the membrane-bound fraction. The pathogenic strain of
C. salmositica had significantly higher total ACP activity than the va
ccine strain and the other 2 species. However, the activity in the pat
hogenic C. salmositica decreased significantly with prolonged in vitro
cultivation. The membrane-bound ACP of the pathogenic C. salmositica
had highest resistance to the ACP inhibitor, sodium tartrate.