2-ETHYL-3-METHYLMALEIMIDE N-BETA-D-GLUCOPYRANOSIDE FROM THE LEAVES OFMANGOSTEEN (GARCINIA-MANGOSTANA)

From a methanolic extract of leaves of Garcinia mangostana, 2-ethyl-3- methylmaleimide N-beta-D-glucopyranoside was isolated by adsorption ch romatography on XAD resin followed by rotation locular countercurrent chromatography. After acetylation, separation was performed by column chromatography on silica gel and preparative HPLC on RP-18 phase. Iden tification was carried out by mass spectrometry (DCI, APCI) and nuclea r magnetic resonance (H-1 NMR, C-13 NMR, H-1 H-1-COSY, HMQC). Assignme nt of the quateranry carbon resonances was achieved by HMBC experiment s. Copyright (C) 1996 Elsevier Science Ltd