SIMPLE DIFFERENTIATION BETWEEN CORE-FUCOSYLATED AND NONFUCOSYLATED GLYCANS BY CAPILLARY ELECTROPHORESIS

Core-fucosylated glycans, derivatized with 2-amino-acridone, consisten tly migrate slower than the corresponding oligosaccharides which lack this fucose residue, using the micellar electrophoretic capillary chro matography conditions outlined in this study. alpha-Fucosidase digesti on of glycans followed by CE analysis has allowed facile differentiati on of these two classes of oligosaccharides and this methodology has b een applied to obtain preliminary information on the carbohydrate cont ent from two glycoproteins, a monoclonal IgG antibody and the soluble complement receptor type 1 (sCR1). (C) 1996 Academic Press, Inc.