SYNTHESIS AND CHARACTERIZATION OF 4-IODOPHENYLBORONIC ACID - A NEW ENHANCER FOR THE HORSERADISH PEROXIDASE-CATALYZED CHEMILUMINESCENT OXIDATION OF LUMINOL

4-Iodophenylboronic acid has been synthesized and shown to be a potent enhancer of the chemiluminescent horseradish peroxidase (Type VI-A)-c atalyzed oxidation of luminol and isoluminol. The enhancer was effecti ve (>100-fold enhancement) in the concentration range 10-1500 mu M. Li ght emission in the presence of the enhancer peaked at 5-10 min after initiation of the reaction and then decayed very slowly. 4-Iodophenylb oronic acid also enhanced reactions catalyzed by horseradish peroxidas e Type IX. The detection limit for Type VI-A horseradish peroxidase wa s 509 amol, and optimum signal enhancement was obtained at 769 mu M co mpared to 154 mu M for 4-iodophenol under the same conditions. (C) 199 6 Academic Press, Inc.