SYNTHESIS OF A CHEMILUMINESCENT PROBE USEFUL FOR THE PURIFICATION OF STEROID 5-ALPHA-REDUCTASE

Authors
GUARNA A POLETTI A CATRAMBONE F DANZA G MARRUCCI A SERIO M CELOTTI F MARTINI L
Citation
A. Guarna et al., SYNTHESIS OF A CHEMILUMINESCENT PROBE USEFUL FOR THE PURIFICATION OF STEROID 5-ALPHA-REDUCTASE, Bioorganic & medicinal chemistry letters, 6(16), 1996, pp. 1997-2002
Citations number
35
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
6
Issue
16
Year of publication
1996
Pages
1997 - 2002
Database
ISI
SICI code
0960-894X(1996)6:16<1997:SOACPU>2.0.ZU;2-4
Abstract
Steroid 5 alpha-reductase is a system of two isozymes (5 alpha R-1 and 5 alpha R-2), which is related to several human diseases. The synthes is of a chemiluminescent probe (AZA-LU) useful for purification of 5 a lpha R is described. AZA-LU has a chemiluminescence detectable up to 1 0(-15) M and is a good inhibitor (IC50 = 0.5 mu M) for rat 5 alpha R-1 expressed in transformed Saccharomicyes cerevisiae yeast. Incubation of AZA-LU with yeast lysates, followed by SDS-PAGE, led to the detecti on of two luminescent protein regions with estimated molecular weights of 26 kDa and 21 kDa. Copyright (C) 1996 Elsevier Science Ltd.