CHARACTERIZATION OF HUMAN PLATELET RECEPTORS FOR ATRIAL-NATRIURETIC-PEPTIDE - EVIDENCE FOR CLEARANCE RECEPTORS

Characterization of human platelet receptors for atrial natriuretic pe ptide (ANP) has been performed using two different methods. First, int racellular cGMP levels were measured after platelet stimulation with ( human)ANP (hANP) and CNP, in order to detect the presence of ANP-A or ANP-B receptors. The intracellular cGMP content was not modified eithe r after hANP or CNP platelet stimulation and thus indicated the lack o f biological active receptors on these cells. Second, displacements of I-125-hANP were similar when induced by hANP or C(4-23)ANP (specific ligand of clearance receptor). Consequently, these results confirmed t hat human platelet membranes bore only clearance receptors for atrial natriuretic peptide.