PRODUCTION, CHARACTERIZATION AND CONTROL OF A NEISSERIA-MENINGITIDIS HEXAVALENT CLASS-1 OUTER-MEMBRANE PROTEIN CONTAINING VESICLE VACCINE

Citation
I. Claassen et al., PRODUCTION, CHARACTERIZATION AND CONTROL OF A NEISSERIA-MENINGITIDIS HEXAVALENT CLASS-1 OUTER-MEMBRANE PROTEIN CONTAINING VESICLE VACCINE, Vaccine, 14(10), 1996, pp. 1001-1008
Citations number
29
Categorie Soggetti
Immunology
Journal title
ISSN journal
0264410X
Volume
14
Issue
10
Year of publication
1996
Pages
1001 - 1008
Database
ISI
SICI code
0264-410X(1996)14:10<1001:PCACOA>2.0.ZU;2-O
Abstract
An experimental serogroup B meningococcal vaccine was prepared from tw o genetically engineered strains; each expressing three different clas s 1 outer membrane proteins (OMPs) (PorA). The two strains expressed t he subtypes P1.7,16,P1.5,2;P1.19,15 and P1.5(c),10;P1.12,13;P1.7(h),4, respectively. Outer membrane vesicles (OMV) were prepared from these strains by deoxycholate extraction, mixed with aluminiumphosphate as a djuvant and formulated to final vaccines. The class 1 OMPs represent c a 90% of the protein in the vaccine. The vaccine was found safe for hu man use and induced a bactericidal immune response in mice against fiv e of the six wild type strains, which served as donors for the various por A genes. Copyright (C) 1996 Elsevier Science Ltd.