The development of techniques for high-speed image acquisition in opti
cal coherence tomography (OCT) systems is essential for suppressing mo
tion artifacts when one is imaging Living systems. We describe a new O
CT system for performing micrometer-scale, cross-sectional optical ima
ging at four images/s. To achieve OCT image-acquisition times of less
than 1 s, we use a piezoelectric fiber stretcher to vary the reference
arm delay. A Kerr-lens mode-locked chromium-doped forsterite laser is
employed as the low-coherence source for the high-speed OCT system. D
ynamic, motion-artifact-fi ee in vivo imaging of a beating Xenopus lae
vis (African frog) heart is demonstrated. (C) 1996 Optical Society of
America