STRUCTURES OF CAULERPA CELL-WALL MICROFIBRIL XYLAN WITH DETECTION OF BETA-1,3-XYLOOLIGOSACCHARIDES AS REVEALED BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY
T. Yamagaki et al., STRUCTURES OF CAULERPA CELL-WALL MICROFIBRIL XYLAN WITH DETECTION OF BETA-1,3-XYLOOLIGOSACCHARIDES AS REVEALED BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY, Bioscience, biotechnology, and biochemistry, 60(8), 1996, pp. 1222-1228
Since the cell wall microfibril xylans from Caulerpa sp, were not assu
med to be constituted from beta-1,3-straight chains of homoxylan like
Bryopsis maxima but were heteropolysaccharides as glucoxylan or branch
ed structures, identification of its fine structures was desired. Alth
ough the results for the linkage analysis were discovered from chemica
l methods, complementary confirmations were attempted by detection of
a series of the beta-1,3-xylooligosaccharides prepared as partial acid
hydrolysates. Complete separation of the higher oligosaccharide in ea
ch was difficult through high performance liquid gel permeation chroma
tography (GPC), so the composition of oligosaccharides in roughly sub-
fractionated samples was detected using matrix-assisted laser desorpti
on ionization/time of flight/mass spectrometry (TOF-MS). By these proc
edures, it was obvious that cell wall xylan from Caulerpa brachypus wa
s constituted from at least 25 xylose residues of linear oligosacchari
des with beta-1,3-linkages. Thus the successive methods of repeating G
PC procedures and TOF-MS spectrometric detection were powerful techniq
ues for the identification of incompletely separated oligosaccharides
and it was available to obtain a series of purified beta-1,3-oligomers
from cell wall xylan.