SENSITIVE ANALYSIS OF PROTEIN PHOSPHATASE INHIBITORS BY THE FIREFLY BIOLUMINESCENCE SYSTEM - APPLICATION TO PP1-GAMMA

Authors
SUGIYAMA Y FUJIMOTO K OHTANI II TAKAI A ISOBE M
Citation
Y. Sugiyama et al., SENSITIVE ANALYSIS OF PROTEIN PHOSPHATASE INHIBITORS BY THE FIREFLY BIOLUMINESCENCE SYSTEM - APPLICATION TO PP1-GAMMA, Bioscience, biotechnology, and biochemistry, 60(8), 1996, pp. 1260-1264
Citations number
25
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
8
Year of publication
1996
Pages
1260 - 1264
Database
ISI
SICI code
0916-8451(1996)60:8<1260:SAOPPI>2.0.ZU;2-G
Abstract
Protein phosphatases are classified into types 1 (PP1) and 2 (PP2A, 2B , and 2C). We have already established a new analysis method for PP2A inhibitors by using the firefly bioluminescence system for detection. This method was successfully applied to determine the PP1 gamma inhibi tory activity of known inhibitors, i.e., calyculin A, microcystin-LR, and tautomycin, requiring less than 10 pmol of a sample.