PROPERTY OF TAKA-AMYLASE-A WITH GLU OR ASP OF THE CATALYTIC RESIDUE REPLACED BY THE CORRESPONDING AMINE

Authors
OYAMA T MYOJIN M NITTA Y TODA H NAGASHIMA T KITAMOTO K
Citation
T. Oyama et al., PROPERTY OF TAKA-AMYLASE-A WITH GLU OR ASP OF THE CATALYTIC RESIDUE REPLACED BY THE CORRESPONDING AMINE, Bioscience, biotechnology, and biochemistry, 60(8), 1996, pp. 1351-1352
Citations number
13
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
8
Year of publication
1996
Pages
1351 - 1352
Database
ISI
SICI code
0916-8451(1996)60:8<1351:POTWGO>2.0.ZU;2-H
Abstract
Compared with the enzyme activity of a recombinant Taka-amylase A (wil d TAA) at 25 degrees C and pH 5.3, those of two mutants (E230Q and D29 7N) were 1/10,000 and 1/16,000 for amylase activity, and 1/920 and les s than 1/20,000 for maltosidase activity, respectively. This indicates that all residual activities of E230Q are not due to contamination by wild-type TAA. The results from difference spectroscopy suggested tha t E230Q retains amylose binding ability.