ENANTIOMERIC SEPARATION BY CAPILLARY ELECTROPHORESIS OF DI-PEPTIDES AND TRI-PEPTIDES DERIVATIZED WITH 9-FLUORENYLMETHYL CHLOROFORMATE USINGVANCOMYCIN AS CHIRAL SELECTOR

Enantiomeric separations of chiral di- and tri-peptides derivatized wi th 9-fluorenylmethyl chloroformate were carried out by capillary elect rophoresis using vancomycin as a chiral selector. The mobilities of va ncomycin were determined in phosphate and HEPES buffer by means of a f actorial design. The zero mobility of vancomycin was found to be, to a small extent, dependent on buffer type, buffer concentration, and the presence of organic modifier. Application of a pH above zero mobility of vancomycin resulted in highly efficient separation of 15 di- and t ri-peptides in less than 12 min. The influence of 2-propanol, buffer t ypes, and sodium dodecyl sulfate on selectivity and efficiency was als o investigated. (C) 1996 John Wiley & Sons, Inc.