TRYPSIN-CATALYZED PEPTIDE-SYNTHESIS WITH VARIOUS P-GUANIDINOPHENYL ESTERS AS ACYL DONORS

Authors
SEKIZAKI H ITOH K TOYOTA E TANIZAWA K
Citation
H. Sekizaki et al., TRYPSIN-CATALYZED PEPTIDE-SYNTHESIS WITH VARIOUS P-GUANIDINOPHENYL ESTERS AS ACYL DONORS, Chemical and Pharmaceutical Bulletin, 44(8), 1996, pp. 1585-1587
Citations number
17
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
Chemical and Pharmaceutical Bulletin → ACNP
ISSN journal
00092363
Volume
44
Issue
8
Year of publication
1996
Pages
1585 - 1587
Database
ISI
SICI code
0009-2363(1996)44:8<1585:TPWVPE>2.0.ZU;2-H
Abstract
Trypsin-catalyzed peptide synthesis has been studied by using p-guanid inophenyl esters of N-alpha-(tert-butyloxycarbonyl)amino acid and pept ide as acyl donor components, The reaction conditions were optimized f or organic solvents, pH, and concentration of acceptor, The method was especially useful for the preparation of various peptides containing D-amino acids, The enzymatic hydrolysis of the resulting products was negligible.