Pm. Jakobs et al., IMMORTALIZATION OF 4 NEW FANCONI-ANEMIA FIBROBLAST CELL-LINES BY AN IMPROVED PROCEDURE, Somatic cell and molecular genetics, 22(2), 1996, pp. 151-157
Fanconi anemia (FA) is an autosomal recessive disease characterized by
birth defects, progressive bone marrow failure and increased risk for
Leukemia. FA cells display chromosome breakage and increased cell kil
ling in response to DNA crosslinking agents. At least 5 genes have bee
n defined by cell complementation studies, but only one of these, FAC
has been cloned to date. Efforts to map and isolate new FA genes by fu
nctional complementation have been hampered by the lack of immortalize
d FA fibroblast cell lines. Here we report the use of a novel immortal
ization strategy to create 4 new immortalized FA fibroblast lines, inc
luding one from the rare complementation group D.