ANTI-CD69 ANTIBODIES ENHANCE PHORBOL-DEPENDENT GLUCOSE-METABOLISM ANDCA2- ANTAGONIST EFFECT OF CYCLOSPORINE-A( LEVELS IN HUMAN THYMOCYTES )

Citation
M. Conde et al., ANTI-CD69 ANTIBODIES ENHANCE PHORBOL-DEPENDENT GLUCOSE-METABOLISM ANDCA2- ANTAGONIST EFFECT OF CYCLOSPORINE-A( LEVELS IN HUMAN THYMOCYTES ), Journal of leukocyte biology, 60(2), 1996, pp. 278-284
Citations number
49
Categorie Soggetti
Immunology,Hematology
ISSN journal
07415400
Volume
60
Issue
2
Year of publication
1996
Pages
278 - 284
Database
ISI
SICI code
0741-5400(1996)60:2<278:AAEPGA>2.0.ZU;2-X
Abstract
The human activation antigen CD69 is an early inducible surface glycop rotein acquired by T cells in the thymus at the stage of positive sele ction and during activation of mature lymphoid cells both in vivo and in vitro, We have studied the regulatory influence of CD69 activation pathway on the glycolytic process and transduction signals of thymocyt es, Treatment of human thymocytes with different anti-CD69 monoclonal antibodies (mAbs), in the presence of submitogenic doses of phorbol es ter, produced an enhanced release of lactate without significant alter ations in Fru 2,6-P-2 levels or phosphofructokinase-2 (PFK-2) and pyru vate kinase activities, A small increase in phosphofructokinase-1 (PFK -1) activity was also detected, Furthemore, anti-CD69 mAb increased th e glucose detritiation from [2-H-3] and [3-H-3]glucose, thus indicatin g an enhanced flux through hexokinase and PFK-1 steps, In addition, de novo synthesis of diacylglycerol slid intracellular Ca2+ levels incre ased after anti-CD69 mAb treatment, The stimulatory effects of anti-CD 69 mAb on both glycolysis and Ca2+ levels were inhibited by cyclospori n A, Because CD69 molecules are present in certain subset populations of immature thymocytes, the ability of anti-CD69 mAb to stimulate the glycolysis, the synthesis of diacylglycerol and the intracellular Ca2 levels suggest that time activation signals delivered through CD69 mo lecules could play a role in the thymus cells maturation.