M. Conde et al., ANTI-CD69 ANTIBODIES ENHANCE PHORBOL-DEPENDENT GLUCOSE-METABOLISM ANDCA2- ANTAGONIST EFFECT OF CYCLOSPORINE-A( LEVELS IN HUMAN THYMOCYTES ), Journal of leukocyte biology, 60(2), 1996, pp. 278-284
The human activation antigen CD69 is an early inducible surface glycop
rotein acquired by T cells in the thymus at the stage of positive sele
ction and during activation of mature lymphoid cells both in vivo and
in vitro, We have studied the regulatory influence of CD69 activation
pathway on the glycolytic process and transduction signals of thymocyt
es, Treatment of human thymocytes with different anti-CD69 monoclonal
antibodies (mAbs), in the presence of submitogenic doses of phorbol es
ter, produced an enhanced release of lactate without significant alter
ations in Fru 2,6-P-2 levels or phosphofructokinase-2 (PFK-2) and pyru
vate kinase activities, A small increase in phosphofructokinase-1 (PFK
-1) activity was also detected, Furthemore, anti-CD69 mAb increased th
e glucose detritiation from [2-H-3] and [3-H-3]glucose, thus indicatin
g an enhanced flux through hexokinase and PFK-1 steps, In addition, de
novo synthesis of diacylglycerol slid intracellular Ca2+ levels incre
ased after anti-CD69 mAb treatment, The stimulatory effects of anti-CD
69 mAb on both glycolysis and Ca2+ levels were inhibited by cyclospori
n A, Because CD69 molecules are present in certain subset populations
of immature thymocytes, the ability of anti-CD69 mAb to stimulate the
glycolysis, the synthesis of diacylglycerol and the intracellular Ca2 levels suggest that time activation signals delivered through CD69 mo
lecules could play a role in the thymus cells maturation.