VISUALIZATION OF PROTEIN-KINASE ACTIVITIES IN SINGLE CELLS BY ANTIBODIES AGAINST PHOSPHORYLATED VIMENTIN AND GFAP

Authors
INAGAKI N TSUJIMURA K TANAKA J SEKIMATA M KAMEI Y INAGAKI M
Citation
N. Inagaki et al., VISUALIZATION OF PROTEIN-KINASE ACTIVITIES IN SINGLE CELLS BY ANTIBODIES AGAINST PHOSPHORYLATED VIMENTIN AND GFAP, Neurochemical research, 21(7), 1996, pp. 795-800
Citations number
30
Categorie Soggetti
Biology,Neurosciences
Journal title
Neurochemical research → ACNP
ISSN journal
03643190
Volume
21
Issue
7
Year of publication
1996
Pages
795 - 800
Database
ISI
SICI code
0364-3190(1996)21:7<795:VOPAIS>2.0.ZU;2-3
Abstract
Vimentin and glial fibrillary acidic protein (GFAP) are intermediate f ilament proteins expressed in the cytoplasm of various types of cells. The head domains of these proteins are phosphorylated by various prot ein kinases. Site- and phosphorylation-specific antibodies which recog nize a phosphorylated serine/threonine residue in the head domains and its flanking sequence provide a useful tool to monitor and visualize protein kinase activities in single cells.