STUDIES ON SOLID-SURFACE ROOM-TEMPERATURE FLUORESCENCE FOR DETERMINATION OF 5 TETRACYCLINE ANTIBIOTICS

The solid surface room temperature fluorescence (SS-RTF) for determina tion of five tetracyclines on filter paper and the simultaneous determ ination of chlortetracycline (CTC) and tetracycline(TC) or CTC and oxy tetracycline were described, Various factors of effect on RTF intensit ies of tetracyclines, such as pH condition, drying temperature and tim e of sample etc, were studied, The linear dynamic ranges, limit of det ections and RSD were: 2.1 similar to 205.0 ng, 1.3 ng and 3.1% for CTC , 5.9 similar to 496.4 ng, 1.8 ng and 2.6% for OTC; 3.8 similar to 481 .0 ag, 3.6 ng and 2.8% for TC; 3.7 similar to 462.6 ng, 2.7 ng and 4.1 % for doxycycline (DOTC); 5.8 similar to 193.0 ng, 5.2 ng and 3.4% for methacycline(MTC), respectively. The recoveries of them in urine are from 95% to 105%.