EFFECT OF GUANIDINE-HYDROCHLORIDE ON THE ACTIVITY OF RNASE-A IN DAB REVERSE MICELLES

The effect of guanidine hydrochloride on the activity of Bovine Pancre atic ribonuclease A solubilized in reverse micelles formed in cyclohex ane by dodecylammonium butyrate (DAB) and water has been investigated using cytidine 2',3'-phosphate as the substrate, It was noted that the enzyme entrapped in DAB reverse micelles maintained more activity com pared to aqueous solution at the same guanidine concentration, because protein molecule was constrained by the micellar environment into a c onformation close to the native, Fluorescence studies showed that the degree of polarization of protein fluorescence in the micelles increas ed with respect to that in water which also suggested a lower mobility of the protein.