PRACTICAL IMPLEMENTATION OF SINGLE-VOXEL DOUBLE-QUANTUM EDITING ON A WHOLE-BODY NMR SPECTROMETER - LOCALIZED MONITORING OF LACTATE IN THE HUMAN LEG DURING AND AFTER EXERCISE

The classical double-quantum editing sequence 90 degrees(x)-tau-180 de grees(y)-tau-90 degrees(x)-t(1)-90 degrees(x)-tau-180 degrees(y)-tau-A Q (tau = 1/4J) was rendered volume selective, by making slice selectiv e the first 90 degrees pulse and the two 180 degrees pulses, Using sim ple rules to ensure optimum radio frequency phase coherence, this sing le-voxel editing sequence, reminiscent of a basic PRESS localization t echnique, was implemented on a whole-body 3 T spectrometer, and in vit ro editing of lactate methyl protons was demonstrated without any sign ificant loss in intrinsic sensitivity, The effectiveness of the propos ed approach in vivo was also illustrated through the localized monitor ing of lactate in the human leg during and after exercise.