MAPPING INTERLEUKIN ENHANCER-BINDING FACTOR-2 GENE (ILF2) TO HUMAN-CHROMOSOME-1 (1Q11-QTER AND 1P11-P12) BY POLYMERASE CHAIN-REACTION AMPLIFICATION OF HUMAN RODENT SOMATIC-CELL HYBRID DNA TEMPLATES

Interleukin-2 (IL-2) is the first lymphokine secreted following T cell activation, Several transcription factors regulate IL-2 gene expressi on, including the nuclear factor of activated T cells (NFAT), NFAT act s at the antigen receptor response element-2 (ARRE-2) sequence in the IL-2 enhancer and is the nuclear target of T cell stimulation signals and the immunosuppressant drugs cyclosporine and FK506, which are pote nt inhibitors of IL-2 gene transcription, NFAT has been cloned and fou nd to consist of two subunits, NF45 (ILF2) and NF90 (ILF3), This commu nication reports the assignment of NF45, interleukin enhancer binding factor 2 gene (ILF2), to human chromosome 1 (1q11-qter and 1p11-p12) b y polymerase chain reaction (PCR) amplification of ILF2-specific DNA s equences from well-characterized human-rodent somatic cell hybrid DNA.