POLYMORPHISM IN MORELS - ISOZYME ELECTROPHORETIC ANALYSIS

The aim of this study was to assess whether isozyme polymorphism in di fferent members of the Morchellaceae could be used to improve the syst ematics in this fungal group and to characterize intraspecific crossin gs between monosporal strains in Morchella esculenta. For this purpose , isozyme electrophoretic analysis of the following enzymes was perfor med: glutamine synthetase, NAD-glutamate dehydrogenase, NADP-glutamate dehydrogenase, aspartate aminotransferase, malate dehydrogenase, NAD- glyceraldehyde phosphate dehydrogenase, glucose phosphate isomerase, a nd superoxide dismutase. The analyses allowed discrimination at the in ter- or intra-specific levels and could help to establish a method of identification for strains in the Morchellaceae. To a certain extent t hey appeared to be suitable to analyze interactions of monosporal stra ins of Morchella esculenta in pairing experiments. The polymorphism sh own in this study was consistent with the phylogenetic relationships b etween the investigated strains only at the genus level.