2-PHOTON AND MULTIPHOTON EXCITATION OF CONJUGATE-DYES USING A CONTINUOUS-WAVE LASER

Authors
HANNINEN PE LEHTELA L HELL SW
Citation
Pe. Hanninen et al., 2-PHOTON AND MULTIPHOTON EXCITATION OF CONJUGATE-DYES USING A CONTINUOUS-WAVE LASER, Optics communications, 130(1-3), 1996, pp. 29-33
Citations number
11
Categorie Soggetti
Optics
Journal title
Optics communications → ACNP
ISSN journal
00304018
Volume
130
Issue
1-3
Year of publication
1996
Pages
29 - 33
Database
ISI
SICI code
0030-4018(1996)130:1-3<29:2AMEOC>2.0.ZU;2-E
Abstract
We discuss the possibility of obtaining a fluorescence signal dependin g nonlinearly on the excitation intensity by inhibiting the fluorescen ce energy transfer in a conjugate-pair dye molecule and its applicatio n in scanning microscopy. The dynamical behaviour of the conjugate dye molecule and the nonlinear behaviour of the fluorescence intensity is analysed. The benefits for scanning fluorescence microscopy are outli ned.