PREPARATIVE ELECTROFOCUSING FOR INDUSTRIAL APPLICATIONS

This paper presents a case study of using a multicompartment isoelectr ic focusing apparatus to determine the isoelectric points and focus pr eparative quantities of brain derived neurotrophic factor (BDNF) and n eurotrophic factor 3 (NT3). A separation of PEGylated from unPEGylated forms of granulocyte colony stimulating factor (G-CSF) is described a s well. Both BDNF and NT3 have substantially higher pi values in this system than is predicted from sequenced,based modeling. Although PEGyl ated forms of G-CSF can be separated from the unPEGylated forms, separ ation of protein with differing degrees of PEGylation was not achieved . The paper additionally demonstrates that this technique can be used simultaneously as an analytical and preparative tool, eliminating the need for analytical IEF gels.