PURIFICATION OF MONOSPECIFIC POLYCLONAL ANTIBODIES FROM HYPERIMMUNE BOVINE WHEY USING IMMUNOAFFINITY CHROMATOGRAPHY

Whey, a by-product of cheese production, is a potential source of prot eins. Immunization of dairy cows in mid-lactation with mouse IgG and d initrophenyl-keyhole limpet hemocyanin resulted in the formation of an tibodies to these antigens in both blood serum and milk. The antibodie s remained in whey during cheese making, and were isolated by immunoaf finity chromatography on matrices with immobilized antigens. The isola ted monospecific antibodies were pure and retained their reactivity to antigens.