SEPARATION AND DETERMINATION OF D-GLUCONIC ACID PRODUCED DURING FERMENTATION BY CAPILLARY ZONE ELECTROPHORESIS

Authors
ZHANG ZP HU ZD YANG GL
Citation
Zp. Zhang et al., SEPARATION AND DETERMINATION OF D-GLUCONIC ACID PRODUCED DURING FERMENTATION BY CAPILLARY ZONE ELECTROPHORESIS, Analytical letters, 29(11), 1996, pp. 2025-2037
Citations number
14
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
29
Issue
11
Year of publication
1996
Pages
2025 - 2037
Database
ISI
SICI code
0003-2719(1996)29:11<2025:SADODA>2.0.ZU;2-B
Abstract
A novel method was developed for separation and determination of D-glu conic acid produced during fermentation by capillary zone electrophore sis (CZE) with direct UV detection at 214 mm, using selected carrier e lectrolyte composed of 6 mM potassium biphthalate, 50 mM disodium hydr ogen phosphate and 15% (v/v) acetonitrile. The effects of concentratio n of phthalate, phosphate and organic modifier (acetonitrile), as well as temperature for the separation were investigated. The method is si mple, inexpensive and will make it very useful in the gluconic acid in dustry.