DETERMINATION OF DRUG-CYCLODEXTRIN BINDING CONSTANTS BY CAPILLARY ZONE ELECTROPHORESIS

Binding constants of the optical isomers of Deprenyl(R) (selegiline) a nd its potential metabolites with (2,6-di-O-methyl)-beta-cyclodextrin were determined using electrophoretic mobility data gained from separa tions performed by capillary electrophoresis, and absorbancies obtaine d from spectrophotometric experiments. To calculate equilibrium consta nts 1:1 complex formation have been assumed. The comparison of the equ ilibrium constants calculated from different methods shows similar val ues in their order of magnitude. Their difference may probably be expl ained by the different media of the measurements. The effect of the st ructure of compounds on chiral discrimination were also elucidated.