EFFECTS OF CAFFEINE ON BONE-CELLS AND BONE-DEVELOPMENT IN FAST-GROWING RATS

Background and methods: Caffeine exerts harmful effects on fetal and s uckling rats, To elucidate the effects of caffeine on bone in growing rats further, dams were fed normal laboratory chow until delivery. At birth, the litters were combined and eight pups were randomly assigned to each dam, Dams with the combined litters were divided into two gro ups, Dams of group 1 received a 20% protein diet as a control, and dam s of group 2 received the 20% protein diet supplemented with caffeine (4 mg/100 g b.w.). Pups from both groups were killed on days 11, 15, 2 2, and 50, Parameters studied were ultrastructure of femoral osteoblas ts, osteocytes, and osteoclasts; the number of osteocytes per area of bone in femoral histological cross sections; structural remodeling and shape of the lateral tibial metaphysis as revealed by scanning electr on microscopy; and plasma Cu and Zn concentrations. Results: Rats in t he caffeine groups had significantly fewer osteocytes per area of femo ral cross section, retarded structural remodeling of the lateral tibia l metaphysis, osteoblasts and osteocytes with disrupted swollen mitoch ondria, and decreased plasma Cu and Zn levels when compared with contr ols. Conclusions: These results indicate that if young, rapidly growin g rats are exposed to caffeine, disruption of osteoblasts and retarded bone development occur, which could be related to the decreased plasm a Cu level in the young animals. (C) 1996 Wiley-Liss, Inc.