PATHOGENESIS OF CLOSTRIDIUM-PERFRINGENS TYPE-C IN EXPERIMENTAL GUINEAPIGS .3. DISEASE PRODUCTION BY WASHED CELLS

Intragastric administration of washed cells of Clostridium perfringens type C and sterile culture medium mixed with trypsin Inhibitor i.e. r aw soybean flour, produced enterotoxaemic infection in guineapigs show ing characteristic necrotising haemorrhagic enteritis along with haemo concentration. leucocytosis. neutrophilia and lymphocytopenia. The rep lacement of raw soybean flour with autoclaved (trypsin-inhibiting acti vity destroyed) product rendered the organisms ineffective. Toxigenesi s and characteristic lesions production suggested the usefulness of gu ineapigs as a model for studying the pathogenesis of C. perfringens ty pe C infection.