Sl. Woo et al., CHARACTERIZATION OF FUSARIUM-OXYSPORUM F-SP PHASEOLI BY PATHOGENIC RACES, VCGS, RFLPS, AND RAPD, Phytopathology, 86(9), 1996, pp. 966-973
Isolates of Fusarium oxysporum were obtained from the vascular tissue
of Phaseolus spp. and characterized by pathogenicity, vegetative compa
tibility, restriction fragment length polymorphisms (RFLPs), and rando
m amplified polymorphic DNA (RAPD) analyses. Eleven isolates were F. o
xysporum f. sp. phaseoli and comprised five pathogenic races. Two race
s, nine vegetative compatibility groups (VCGs), and three heterokaryon
self-incompatible isolates are new reports for this forma specialis.
Pathogenic and nonpathogenic isolates were not found in the same VCGs
and, in some cases, there was a correspondence of VCG to race and plac
e of origin. RFLPs with five different restriction enzymes and RAPD wi
th four different primers indicated genetic diversity in F. oxysporum
f. sp. phaseoli. The banding patterns showed a correspondence to the V
CGs, but not to the pathogenic races. RFLPs and RAPD markers could be
used to distinguish isolates that were nonpathogenic, self-incompatibl
e, and from obscure origins. The use of all four methods combined was
effective in characterizing and providing insight into the complex rel
ationship of F. oxysporum f. sp. phaseoli.