MOUSE IL-17 - A CYTOKINE PREFERENTIALLY EXPRESSED BY ALPHA-BETA-TCR-CD8- T-CELLS(CD4)

A novel cytokine originally designated murine CTLA-8 was described as a cDNA isolated from an activated T cell hybridoma produced by fusing a mouse cytotoxic T cell clone and a rat T lymphoma, This cDNA, which contains mRNA instability sequences characteristic of many cytokines, encoded a putative secreted protein that was homologous to the ORF13 g ene of Herpesvirus saimiri. The human homolog to this molecule has rec ently been identified as the proinflammatory cytokine IL-17, We descri be the isolation of a cDNA encoding mouse IL-17 from a cDNA library ge nerated from alpha beta TCR+CD4-CD8- thymocytes using a subtraction te chnique that enriched for activation specific genes, This cDNA shares 87.3% amino acid identity to the previously described murine CTLA-8, C omparison of murine CTLA-8 to a cDNA we isolated from activated rat sp lenocytes revealed that murine CTLA-8 is, in fact, the rat homolog of IL-17, Mouse IL-17 mRNA is specifically expressed by activated alpha b eta TCR+CD4-CD8- T cells, a small subset with a potentially important role in immune regulation, Mouse, rat, and human IL-17 can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can re cognize the mouse receptor.