ISOLATION OF DNA-SEQUENCES AMPLIFIED AT CHROMOSOME 19Q13.1-Q13.2 INCLUDING THE AKT2 LOCUS IN HUMAN PANCREATIC-CANCER

In the human pancreatic cancer cell line PANC1, we detected several DN A fragments with abnormally intensified signals by restriction landmar k genomic scanning. Major five of these fragments were cloned. All of the cloned fragments were mapped at the 19q13.1-13.2 region where the AKT2 oncogene was located. Southern blotting using the cloned DNA frag ments and a fragment of AKT2 cDNA as probes revealed that the AKT2 gen e was amplified in 3 of 12 pancreatic cancer cell Lines analyzed inclu ding PANC1 and in 3 of 20 primary pancreatic cancers. The AKR gene was overexpressed in the 3 cell lines with the amplified gene. The result s suggest that the AKT2 gene is a candidate oncogene activated by ampl ification in some human pancreatic cancers. (C) 1996 Academic Press, I nc.