T. Yasuda et al., A NEW INDIVIDUALIZATION MARKER OF SWEAT - DEOXYRIBONUCLEASE-I (DNASE-I) POLYMORPHISM, Journal of forensic sciences, 41(5), 1996, pp. 862-864
We have confirmed for the first time, both biochemically and immunolog
ically, the existence of deoxyribonuclease I (DNase I) in human liquid
sweat. Isoelectric focusing of sweat samples on polyacrylamide gels (
pH 3.5 to 5), followed by dried agarose film overlay detection, was us
ed to determine the phenotypes of sweat DNase I. Because this detectio
n method not only had high sensitivity, but also high band resolution,
it was possible to determine DNase I types from sweat samples of 50 t
o 100 mu L. Pretreatment of sweat samples with sialidase was essential
for typing to enhance markedly the sensitivity accompanied by simplif
ication of the isozyme pattern. The DNase I types in all sweat samples
were consistently related to the types found in corresponding blood,
urine, and semen samples. DNase I typing could, therefore, provide a n
ovel. discriminant characteristic in the forensic examination of sweat
.