USE OF A NAPHTHYLETHYLCARBAMOYLATED-BETA-CYCLODEXTRIN CHIRAL STATIONARY-PHASE FOR THE SEPARATION OF DRUG ENANTIOMERS AND RELATED-COMPOUNDS BY SUBCRITICAL AND SUPERCRITICAL FLUID-CHROMATOGRAPHY

Enantiomeric separation of a variety of drugs and related compounds wa s achieved on an (S)-naphthylethylcarbamoylated-beta-cyclodextrin (S-N EC-CD) chiral stationary phase (CSP) using sub- and supercritical flui d chromatography (SFC). Compounds previously resolved on native or der ivatized cyclodextrin CSPs in liquid chromatography (LC) using reverse d phase or polar organic mobile phase modes could be resolved in SFC u sing a simple carbon dioxide/methanol eluent, Resolution of cromakalim , which is not possible on the S-NEC-CD column in LC, was readily acco mplished in SFC. The importance of modifier, temperature, and pressure was assessed in relation to retention, selectivity, and resolution. T he nature of the modifier and the modifier concentration were found to be crucial parameters. (C) 1996 Wiley-Liss, Inc.