IDENTIFICATION OF RAPD MARKERS LINKED TO LINOLENIC ACID GENES IN RAPESEED

Authors
JOURDREN C BARRET P HORVAIS R DELOURME R RENARD M
Citation
C. Jourdren et al., IDENTIFICATION OF RAPD MARKERS LINKED TO LINOLENIC ACID GENES IN RAPESEED, Euphytica, 90(3), 1996, pp. 351-357
Citations number
32
Categorie Soggetti
Plant Sciences",Agriculture
Journal title
Euphytica → ACNP
ISSN journal
00142336
Volume
90
Issue
3
Year of publication
1996
Pages
351 - 357
Database
ISI
SICI code
0014-2336(1996)90:3<351:IORMLT>2.0.ZU;2-3
Abstract
The inheritance of the low linolenic acid content (derivated from muta nt lines) in rapeseed was investigated. Molecular techniques of gene m apping through RAPD markers were applied on a microspore-derived proge ny from a high x low linolenic acid Fl hybrid. 'Bulked segregant analy sis' made it possible to test rapidly number of RAPD primers. Two link age groups of 6 markers (72.7 cM and 75.6 cM) were determined. Each co rresponded to a major QTL which explained 24% and 30.7% of the total p henotypic variation of the linolenic acid content. It was confirmed th at two independant mutations were implied in the low linonenic acid co ntent.