AN INTENSELY LUMINESCENT POLYMERIC LANTHANIDE CHELATOR FOR MULTIPLE FLUORESCENCE LABELING OF BIOMOLECULES

A new bifunctional chelating agent has been synthesized and used to pr epare a stable, intensely luminescent polymer, PLCS-Eu(III) with an ex tinction coefficient of 10(+6) (M-cm)(-1), a very large stokes shift ( Ex 337, Em 617nm), a 5nm emission bandwidth and a quantum yield of 0.6 . The PLCS-Eu(III) complex was found stable towards EDTA and prolonged irradiation It was coupled, as a coating, upon 3 mu m beads which in bun were coupled to oligonucleotides, for use in a nucleic acid sandwi ch assay. Its millisecond emission lifetime will allow it to be used f or time resolved fluorescence detection. PLCS-Eu(III), a novel chelato r, may be a useful new tool for array based studies of biopolymer inte ractions. Copyright (C) 1996 Elsevier Science Ltd