Jb. Lamture et al., AN INTENSELY LUMINESCENT POLYMERIC LANTHANIDE CHELATOR FOR MULTIPLE FLUORESCENCE LABELING OF BIOMOLECULES, Tetrahedron letters, 37(36), 1996, pp. 6483-6486
A new bifunctional chelating agent has been synthesized and used to pr
epare a stable, intensely luminescent polymer, PLCS-Eu(III) with an ex
tinction coefficient of 10(+6) (M-cm)(-1), a very large stokes shift (
Ex 337, Em 617nm), a 5nm emission bandwidth and a quantum yield of 0.6
. The PLCS-Eu(III) complex was found stable towards EDTA and prolonged
irradiation It was coupled, as a coating, upon 3 mu m beads which in
bun were coupled to oligonucleotides, for use in a nucleic acid sandwi
ch assay. Its millisecond emission lifetime will allow it to be used f
or time resolved fluorescence detection. PLCS-Eu(III), a novel chelato
r, may be a useful new tool for array based studies of biopolymer inte
ractions. Copyright (C) 1996 Elsevier Science Ltd