P-SELECTIN AND E-SELECTIN MEDIATE RECRUITMENT OF T-HELPER-1 BUT NOT T-HELPER-2 CELLS INTO INFLAMED TISSUES

WHEN activated, T helper cells differentiate into one of two subsets, Th1 and Th2, characterized by distinct profiles of cytokine production . Th1 cells activate pro-inflammatory effector mechanisms involved in protection and autoimmunity, whereas Th2 cells induce humoral and alle rgic responses and downregulate local inflammation(1,2). Apart from di fferences in the repertoire of cytokines, no phenotypic attributes are established that distinguish the two subsets. Here we show that Th1 c ells, but not Th2 cells, are able to bind to P-selectin and E-selectin . Moreover, only Th1 cells can efficiently enter inflamed sites in Th1 -dominated models, such as sensitized skin or arthritic joints, but no t in a Th2-dominated allergic response, Immigration of Th1 cells into inflamed skin can be blocked by antibodies against P- and E-selectin, These results provide evidence for adhesion mechanisms to distinguish between the two T helper subsets and mediate their differential traffi cking, They indicate that selective recruitment is an additional level of regulation for both effector function profile and character of a l ocal immune response.