SEPARATION OF POLYAROMATIC COMPOUNDS BY L IQUID-CHROMATOGRAPHY USING CELLULOSE 3,5-DINITROBENZOATE AS THE PACKING MATERIAL

Cellulose 3,5-dinitrobenzoate (DNB-cellulose) with degree of substitut ion (DS) up to 2.65 was synthesized by the reaction of microcrystallin e cellulose with 3,5-dinitrobenzoyl chloride. The application of the D NB-cellulose to a liquid chromatography as a packing material was inve stigated in order to separate the five polyaromatic compounds: naphtha rene, anthracene, phenanthrene, pyrene, and chrysene. The mutual separ ation ability of the packing material for the five samples has been fo und to be dependent on the DS by 3,5-dinitrobenzoyl group. In this stu dy the optimum DS was about 0.21 in the case using dichloromethane as the eluent, The effect of eluent composition was also investigated for the separation of the five polyaromatic compounds. The five samples w ere successfully separated from each other when the mixture of acetoni trile, dioxane and dichloromethane (5 : 5 : 90 in vol,) was used as th e eluent. The formations of charge transfer complex between DNB-cellul ose and the polyaromatic compounds were deduced from the analysis of U V spectra of the mixture of methyl 3,5-dinitrobenzoate as a model comp ound of DNB-cellulose and the polyaromatic compounds in dichloromethan e.