EFFECTS OF CACN2 TREATMENT OF SEEDS OF INUBIE (ECHINOCHLOA-ORYZICOLA)ON SYNTHESIS OF PROTEIN AND RNA DURING GERMINATION

Protein and RNA induction during the germination of Inubie (Echinochlo a oryzicola) seeds pretreated with CaCN2 was studied, It was observed that the germination of Echinochloa oryzicola seeds was delayed after pretreatment with a high concentration of CaCN2 for 18 h. A considerab le difference in the protein patterns was detected in the SDS-PAGE gel between the control (H2O) and CaCN2 treatment, particularly a 48 kilo dalton (kD) protein band disappeared in CaCN2 treatment. At the tested three levels of CaCN2, the induction of this 48 kD protein was appare ntly inhibited during the germination process even when the duration o f the pretreatment was as short as 2 h. When imbibed with water, the a mount of the 48 kD protein increased rapidly within 1 h from the trace level in the dry seeds, and reached the maximum level after about 1-2 d. The results showed that the CaCN2 treatment also decreased the tot al RNA level in the germinating seeds, The relation of this protein in duction and RNA decline with the beginning of Echinochloa oryzicola ge rmination was discussed.