Xl. Ren et al., POLY(ETHYLENE-PROPYLENE GLYCOL)-MODIFIED FUSED-SILICA COLUMNS FOR CAPILLARY ELECTROPHORESIS USING EPOXY-RESIN AS INTERMEDIATE COATING, Journal of chromatography, 741(1), 1996, pp. 115-122
Citations number
28
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
An epoxy resin was used for fused-silica capillary column pretreatment
before coating with a hydrophilic polymer for capillary electrophores
is. The unique properties of the epoxy resins allow the deposition of
a dense polymer layer on the column surface using the dynamic coating
method. By using a free-radical cross-linking initiator, a hydrophilic
polymer coating can be subsequently immobilized over the resin covere
d surface. The coating is of sufficient thickness and hydrophilicity t
o reduce protein adsorption and minimize electro-osmotic flow. Basic p
rotein separations were achieved with high efficiency and reproducibil
ity; one thousand consecutive runs on a single column gave an R.S.D. v
alue of less than 2% in migration time. The coating was stable through
out the pH range 4-11. The coating provides a simple, stable, and repr
oducible means for deactivation of fused-silica columns for capillary
electrophoresis.