CYTOPLASMIC CREB-ALPHA-LIKE ANTIGENS IN SPECIFIC REGIONS OF THE RAT-BRAIN

Citation
O. Hermanson et al., CYTOPLASMIC CREB-ALPHA-LIKE ANTIGENS IN SPECIFIC REGIONS OF THE RAT-BRAIN, Biochemical and biophysical research communications, 225(1), 1996, pp. 256-262
Citations number
30
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
225
Issue
1
Year of publication
1996
Pages
256 - 262
Database
ISI
SICI code
0006-291X(1996)225:1<256:CCAISR>2.0.ZU;2-#
Abstract
We have investigated the expression of the alpha-region of CREB (amino acids 88-101) in the rat brain. This region is encoded by a separate exon and has been reported to enhance the transcriptional efficiency o f CREB. Light microscopic immunohistochemistry showed labeled neurons in several brain regions, such as the spinal cord, pontine reticular f ormation, cerebellum, hypothalamus, hippocampus and olfactory cortex. The labeling was exclusively cytoplasmic, and electron microscopy reve aled that the CREB alpha-immunoreactivity was localized to the Golgi a pparatus and axon terminals. Immunoblotting after SDS-PAGE showed spec ifically detected proteins of 21 and 160 kD. We suggest that neuronal CREB alpha-proteins represent alternatively spliced isoforms of CREB t hat lack the nuclear translocation signal. These isoforms may be invol ved in the regulation of CREB-mediated transcription. (C) 1996 Academi c Press, Inc.