PROMOTER STRUCTURE OF THE MOUSE TATA-BINDING PROTEIN (TBP) GENE

5'-RACE and genomic cloning were used to determine that the mouse TBP (mTBP) gene consists of one 5'-terminal non-coding exon followed by se ven protein-coding exons. The region upstream of the first exon lacks a TATA-box. Hence, as with the case of other genes carrying TATA-less promoter, transcription starts from a cluster of sites which are locat ed at the restricted region of mTBP gene. Interestingly, sequences of this region are well conserved between human and mouse TBP genes, sugg esting that both mouse and human TBP genes drive mRNA synthesis in a s imilar way, and the sequence homology between two genes was used to as sign a putative start point for the human TBP gene, Mouse, human, and Trimersurus gramineus (green habu snake) TBP genes share two GC-rich r egions in their promoter regions. Thus, it is probable that diverse sp ecies of vertebrates commonly use TATA-less promoter bearing GC-rich r egions to direct ubiquitous TBP expression. (C) 1996 Academic Press, I nc.