PROMOTER STRUCTURE OF TGF-BETA TYPE-II RECEPTOR REVEALED BY OLIGO-CAPPING METHOD AND DELETION ANALYSIS

The promoter of TGF-beta type II receptor lacks TATA box or CAAT box, In order to define the exact transcriptional start site(s), we used '' oligo-capping'' method developed by ourselves, The major transcripts w ere started from GAA located between -35 and -33 relative to 5' end of cDNA. Other minor transcripts started from -4, +11 and +17. Deletion analysis of this promoter region revealed that it contained two adjace ntly located promoters (Pi and P2) capable of acting by itself. The P1 is located between -22 and +55 which covers the minor transcriptional start sites described above. The P2 is located just upstream of the P 1 between -137 and -22. The results showed all of the transcripts were started either from P1 or P2. (C) 1996 Academic Press, Inc.