S. Lasry et al., INTERSTRAIN VARIATIONS IN THE CYTOMEGALOVIRUS (CMV) GLYCOPROTEIN-B GENE SEQUENCE AMONG CMV-INFECTED CHILDREN ATTENDING 6 DAY-CARE-CENTERS, The Journal of infectious diseases, 174(3), 1996, pp. 606-609
Using the shell vial assay and sequence analysis of a variable region
of the glycoprotein B (gB) gene, cytomegalovirus (CMV) excretion rates
in urine and virus transmission were studied among 93 children from s
ix day care centers (DCCs), During a 6-month period, excretion rates d
iffered significantly between DCCs (P < .001), The 6 gB gene sequences
, obtained from 24 CMV-infected children, were classified in four prev
iously defined groups, In five DCCs, 2 or 3 strains cocirculated, and
none was dominant, Infection could have been acquired outside the DCC
for 2 children and inside it for 9. Two children from the same DCC had
mixed infections. No differences in hygiene, child care practices, or
experience and level of qualification of the staff could explain this
wide variety of excretion rates between DCCs, The distribution of gB
gene patterns observed does not suggest that 1 type was dominant or mo
re efficiently transmitted.