PROTECTION FROM PRECONDITIONING CAN BE REINSTATED AT VARIOUS REPERFUSION INTERVALS

Citation
Ek. Iliodromitis et al., PROTECTION FROM PRECONDITIONING CAN BE REINSTATED AT VARIOUS REPERFUSION INTERVALS, Cardiovascular drugs and therapy, 10(3), 1996, pp. 341-346
Citations number
22
Categorie Soggetti
Pharmacology & Pharmacy","Cardiac & Cardiovascular System
ISSN journal
09203206
Volume
10
Issue
3
Year of publication
1996
Pages
341 - 346
Database
ISI
SICI code
0920-3206(1996)10:3<341:PFPCBR>2.0.ZU;2-F
Abstract
The aim of this study was to evaluate the way in which short-term prot ection declines and is eventually lost in preconditioning and to deter mine the efficacy of a second preconditioning at various reperfusion i ntervals. Male rabbits were divided into six groups. Forty-five minute s (sustained) ischemia followed by 120 minutes reperfusion was applied 60, 65, 70, 75, and 80 minutes after a 5 minute preconditioning (grou ps A, B, C, D, and E) and in a control group (F) after no precondition ing. The infarct to risk ratio (IIR) was 38.3 +/- 3.5% in group A, 46. 0 +/- 7.8% in B, 61.6 +/- 9.7% in C, 68.1 +/- 4.2% in D, 64.5 +/- 7.8% in E, and 61.0 +/- 7.7% in F. Group A had a smaller IIR compared with groups C, D, E, and F (p < 0.05), In another series, groups G, H, and I were exposed to two 5-minute preconditioning stimuli, separated, re spectively, by 45, 60, and 75 minutes of reperfusion; 10 minutes after the last preconditioning, the animals were exposed to 45-minutes isch emia and 120 minutes reperfusion. Groups A and D (with the smaller and higher IIR ratio) were also incorporated into this protocol in order to compare the effect of the additional preconditioning with the singl e one. The IIR ratio was 25.4 +/- 8.5% in group G, 22.8 +/- 7.0% in gr oup H, and 14.7 +/- 4.0% in group I(p = NS). Group D showed a higher I IR compared with groups G, A, and H (p < 0.01), acid group I had a sma ller I/R compared with groups A (p < 0.01) and D (p < 0.001). Cardiopr otection after a first preconditioning declines gradually and is event ually lost. An additional preconditioning is always effective, and the longer the interval from the first preconditioning, the more potent i s the effect.