Bv. Worgul et al., USE OF SUBJECTIVE AND NONSUBJECTIVE METHODOLOGIES TO EVALUATE LENS RADIATION-DAMAGE IN EXPOSED POPULATIONS - AN OVERVIEW, Radiation and environmental biophysics, 35(3), 1996, pp. 137-144
Citations number
58
Categorie Soggetti
Biophysics,"Radiology,Nuclear Medicine & Medical Imaging","Environmental Sciences
The general epidemiological acceptability of prevalence, or incidence,
for assessing risk of radiation cataract development has dictated an
almost exclusive dependence on cataract onset as a measure of cataract
ogenicity for given doses of radiation. The advent of instrumentation
capable of acquiring images amenable to quantitative analyses offers t
he possibility of exploiting ''relative opacification'' as an added, i
f not exclusive, parameter. This development is particularly important
in efforts to assess populations such as that in the Altai, which are
temporally far removed from their exposure and among whom there exist
s a large subset with extant cataracts. The new technologies, Scheimpf
lug and retroillumination imaging, combined with the application of th
e appropriate analytical algorithms can not only provide quantitative
and nonsubjective assessment of lens transparency, but also serve as a
means to immortalize the state of the pathology at the time of acquis
ition. Highly relevant to the assessment of an aging exposed populatio
n is the use of lens epithelial fragments as potential dosimeters. The
material is routinely available as a result of cataract extraction pr
ocedures and is amenable to the application of a modified micronucleus
(MN) assay. The MN assay in the lens has tremendous advantages over i
ts use in other tissues for a number of reasons, not least of which is
that lens MNs are extremely long-lived. Given the relative ease of ap
plication and its potential as a radiation bioindicator, the lens MN a
ssay should be considered in any follow-up of populations exposed to i
onizing radiation.