EXPRESSION OF SEROTONIN RECEPTORS IN HUMAN FETAL ASTROCYTES AND GLIOMA CELL-LINES - A POSSIBLE ROLE IN GLIOMA CELL-PROLIFERATION AND MIGRATION

Expression of seven serotonin or 5-hydroxytryptamine (5-HT) receptors (5-HT1D alpha, 5-HT1E, 5-HT2, 5-HT1A, 5-HT1C, 5-HT1D beta, and 5-HT6) was investigated in human normal fetal astrocytes and eight glioma cel l lines by reverse transcription and polymerase chain reaction (RT-PCR ). No expression of 5-HT1D beta and 5-HT6 was observed in any of the c ell lines studied. The 5-HT1D alpha receptor was found to be expressed in two human glioma cell lines but not in normal astrocytes. In addit ion, only three glioma cell lines expressed the 5-HT1E receptor. The 5 -HT1C receptor was expressed in six glioma cell lines but not in norma l astrocytes while the 5-HT1A was found to be expressed in normal astr ocytes from the left hemisphere and in six glioma cell lines but not i n normal astrocytes from the cerebellum. Interestingly, the 5-HT2 rece ptor was expressed in all cells studied but very weakly in normal astr ocytes. The effect of 5-HT on glioma cell proliferation, migration, an d invasion was also investigated. Serotonin was found to positively mo dulate these three processes in vitro. These results suggest that 5-HT may play an important role in the control of the biological propertie s of human glioma cells.