A. Murakami et al., 1'-ACETOXYCHAVICOL ACETATE, A SUPEROXIDE ANION GENERATION INHIBITOR, POTENTLY INHIBITS TUMOR PROMOTION BY 12-O-TETRADECANOYLPHORBOL-13-ACETATE IN ICR MOUSE SKIN, Oncology, 53(5), 1996, pp. 386-391
The anti-tumor-promoting activity of 1'-acetoxychavicol acetate (ACA)
was examined in a two-stage carcinogenesis experiment in ICR mouse ski
n using 7,12-dimethylbenz[a]anthracene (0.19 mu mol) and 12-O-tetradec
anoylphorbol-13-acetate (TPA; 1.6 nmol). Topical application of ACA (1
60 nmol) markedly reduced the average number of tumors per mouse and t
he ratio of tumor-bearing mice: inhibition ratios 90% (p < 0.001) and
42% (p < 0.005), respectively. ACA even at a dose equimolar to TPA(1.6
nmol) significantly reduced the average number of tumors per mouse: i
nhibitory ratio 44% (p < 0.05). ACA potently inhibited TPA-induced sup
eroxide (O-2(-)) generation in differentiated HL-60 cells (IC50 = 4.3
mu M) and suppressed the lipid hydroperoxide formation by 42% (p < 0.0
01) in the ethyl linoleate autoxidation test.