EXPRESSION PATTERNS OF ACIDIC AND BASIC FIBROBLAST GROWTH-FACTOR IN LOACH FISH EMBRYOS

Heparin-binding fractions were taken from the heparin sepharose column s on which extracts of leach fish (Misgurnus anguillicaudatus) embryos from blastula, gastrula, 4-8 and 12-16 somites stages were applied. T hese heparin-binding fractions, except the fraction derived from 12-16 somite embryos, showed potent mitogenic activity on fibroblast-like c ells derived from caudal fin blastema of goldfish. Western blot analys is of these heparin-binding fractions was carried out using monoclonal antibodies against human acidic and basic fibroblast growth factors ( FGF-1 and -2). An immunoreactive FGF-1 band at 16 kD was detected in t he heparin-binding fraction derived from embryos in each stage of blas tula, gastrula and 4-8 somites. An immunoreactive FGF-2 band at 17 kDa was detected exclusively in the heparin-binding fraction derived from 4-8 somite embryos. In the heparin-binding fraction derived from 12-1 6 somite embryos neither immunoreactive FGF-1 nor -2 member band was d etectable.